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Morbid and DNA Fragmented Sperm Depletion Nanoparticles

Nano-sized crystals form super-paramagnetic particles coated with glycoproteins.  The particles are highly effective at depleting morbid/DNA fragmented spermatozoa prior to Intra Cytoplasmic Sperm Injection (ICSI) and in vitrofertilization (IVF).

Applications

  • Male factor with high DFI undergoing ICSI or IVF.
  • Male factor with high DFI undergoing IUI. 
  • Recurrent miscarriage that can be attributed to male factor. 
  • Early pregnancy loss attributed to male factor.

According to latest study conducted on sperm selection by magnetic nano-particles, the percentage of high-quality embryos, pregnancy and implantation rates was significantly higher in the treated groups compared to Density Gradient Centrifugation (DGC) alone.

(Hum Fertil (Camb). 2018 Jan 24:1-8. doi: 10.1080/14647273.2018.1424354.)

Particle Characteristics

  • Irregular particle shape, expanded surface area.
  • Compatible with flow cytometry, PCR & F.I.S.H. 
  • Rapid magnetic response.
  • Efficient size, visible under the microscope, thereby avoiding non-specific binding on viable cells.
  • Preserved in Sodium Azide for maximum sterilization (optional).

Particle Advantages

  • Ease of use; with washed sperm cells, additional process step DGC may not be necessary.
  • Gentle separation, minimizing risk of cell damage and shearing of nucleic acids.
  • No magnetic columns required.
  • No pre-conditioning of particle or sperm sample.
  • No special reagents required.
  • Volume processing capability from micro-liters to liters.

Effectiveness of magnetic particles shown in Graph 1. on DNA Fragmentation,  and Graph 2. on Hyperactivation

Pregnancy Rates: Comparing success rates for MCRM Fertility vs other clinics not using the same technology in the same state.

Recent success rates across the state of Illinois comparing one center using our technology in sperm selection (MCRM Fertility clinic) vs. other clinics in the same state. The Centers for Disease Control and Prevention (CDC) compiles and publishes data regarding individual assisted reproductive technology (ART) centers as well as Society for Assisted Reproductive Technologies (SART).

General resources regarding the technology:

Effects of magnetic-activated cell sorting on sperm motility and cryosurvival rates: https://www.ncbi.nlm.nih.gov/pubmed/15866582

Clinical outcomes of magnetic activated sperm sorting in infertile men candidate for ICSI: https://www.ncbi.nlm.nih.gov/pubmed/29363381

Magnetic-activated cell sorting for sperm preparation reduces spermatozoa with apoptotic markers and improves the acrosome reaction in couples with unexplained infertility: https://www.ncbi.nlm.nih.gov/pubmed/20150175

Clinical outcomes of magnetic activated sperm sorting in infertile men candidate for ICSI: https://www.ncbi.nlm.nih.gov/pubmed/20150175

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